Bxb1-mediated recombination guide

INTRODUCTION

Here we describe the general procedure for using Bxb1 to integrate a pre-fabricated cassette into a MIN-tag locus as well as how to screen for positive recombinations. This step requires a cassette harboring an attB sequence. This can be done using one of the many already available constructs from the MIN Toolbox. For designing and producing your own attB constructs, see the Cloning guide.
For more information see Mulholland et al. 2015

Detailed instructions and protocols can be found HERE